Detecting Cry1Ab gene in Mon0810 Transgenic Maize by PCR Lab Report

Detecting Cry1Ab gene in Mon0810 Transgenic Maize by PCR - Lab Report Example The idea is to check for the gene size of Cry1Ab in order to distinguish it from the rest of the other gene strands. Cry1Ab is a gene from an incorporated plant pesticide thus its genetic sequence is known. A comparison of the known and the established one from the photograph helps to determine its presence in the transgenic maize. If its size is seen to match with the known then it is present, if there is no matching band of close size then Cry1Ab is not present in the maize. Basically, PCR is an easier method for detection of genes as their amplification and comparison with the known size of the DNA strand becomes captured. GAMARRA,LUIS FERNANDO RIMACHI, DELGADO,JORGE ALCAÃŒ NTARA, VILLASANTE,YENY AQUINO, & ORTIZ,RODOMIRO. (2011). Detecting adventitious transgenic events in a maize center of diversity. Electronic Journal of Biotechnology. Electronic Journal of Biotechnology. http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400009. WALSH, MARIA C., BUZOIANU, STEFAN G., GARDINER, GILLIAN E., REA, MARY C., GELENCSÉR, EVA, Jà NOSI, ANNA, EPSTEIN, MICHELLE M., ROSS, R. PAUL, & LAWLOR, PEADAR G. (n.d.). Fate of Transgenic DNA from Orally Administered Bt MON810 Maize and Effects on Immune Response and Growth in Pigs. Public Library of Science.